Protoplast Regeneration in Clostridium tertium: Isolation of Derivatives with High-Frequency Regeneration.

نویسندگان

  • S Knowlton
  • J D Ferchak
  • J K Alexander
چکیده

A procedure was developed for the formation and regeneration of protoplasts in Clostridium tertium. Although in the parent strain most of the protoplasts gave rise to L-form colonies, derivatives could be isolated which exhibited high regeneration frequencies.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

SHOOT REGENERATION FROM SAFFRON PROTOPLASTS IMMOBILIZED IN Ca-ALGINATE BEADS

Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...

متن کامل

PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR DELAWARE USING SILVER THIOSULFATE (STS)

The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...

متن کامل

Protoplast formation and cell wall regeneration in Clostridium perfringens.

A protocol was developed for the efficient production and regeneration of Clostridium perfringens protoplasts. Cell wall regeneration frequencies of up to 5% were obtained.

متن کامل

Clostridium acetobutylicum Protoplast Formation and Regeneration.

Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described.

متن کامل

Efficient Protocol for Protoplast Isolation and Plant Regeneration of Fritillaria imperialis L

The present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of Fritillaria imperialis L. There is no published method recommended for protoplast isolation and regeneration from Fritillaria imperialis L. A range of factors, which influence the success of isolation and regeneration of F. imperialis protoplasts, were investigated. From the results obta...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 48 6  شماره 

صفحات  -

تاریخ انتشار 1984